UV Vis spectroscopy

UV Vis spectroscopy is based on the interaction of light with matter in the UV to visible spectral range, with energy such that electronic transitions are promoted in samples. The phenomena that can observed are reflection, transmission, scattering and absorption of radiation by the material examined. The measurement of quantities to describe these phenomena allows the characterisation of samples with regard to optical properties, like refractive index, transmittance, reflectance, dielectric function.
The possibility of identifying substances by their typical characteristic absorption patterns and evaluating their concentration makes this technique very popular in industry and research in various fields (chemical, pharmaceutical, biological, agri-food…). Furthermore, the measurement of optical properties is of primary importance for the study of advanced materials, such as those used for opto-electronics, photovoltaics, and sensor technology.
In our laboratories, UV-Vis spectroscopy is a fundamental technique in research involving the study of the structure of organic and inorganic solids (using high-performance UV-Vis spectrometers), the analysis of biofluids for the study of materials for optics and optometry (with microvolume spectrometer) and the study of the chiral properties of biomolecules (via spectropolarimeter).

Our instrumentation

The Network includes a large collection of multi-equipped spectrophotometers, a spectropolarimeter for circular dichroism measurements, and a spectrophotometer for microvolume analysis.

High-performance spectrophotometers

Lambda 1050+ Perkin Elmer

Double-beam UV-Vis-NIR spectrophotometer for transmission and reflection optical spectroscopy measurements of solid samples.
Technical specifications:
● spectral range: 190 nm – 3300 nm;
● double monochromator: each equipped with two holographic gratings;
● bandwidth: 0.05 – 5.00nm (UV/Vis); 0.2 – 20 nm (NIR);
● detection system: three detectors (PMT, InGaAs and PbS), with photometric limit 10 absorbance.
Available configurations:
● transmission measurements at normal and oblique incidence (angle of incidence: -75°/+75°);
● absolute specular reflectivity measurements at near-normal incidence (incidence angle ~ 7.5°);
● specular reflectivity measurements relative to oblique incidence (angle of incidence: 15°/60°);
● transmission (at normal and oblique incidence) and absolute specular reflectivity (at near- normal incidence) measurements at low temperature (down to 7 K);
● measurements in linearly polarised light (spectrophotometer equipped with depolariser and Glan-Taylor polarisers);
● diffuse light intensity measurements in transmission and/or reflection with a 10 cm diameter integrating sphere (spectral range: 250 nm – 2500 nm, photometric limit: 2/3 absorbance) equipped with two detectors (PMT and InGaAs ) and accessory for reflection measurements on powders and samples smaller than 1 cm2.
Applications:
study of optical properties and the optical-structure relationship in single crystals and thin films of organic molecular materials for applications in the field of organic electronics and photonics;
● optical characterisation of inorganic powders;
● study of organic and inorganic, crystalline, amorphous or nanostructured solids;
● optical characterisation of samples (e.g. pigments, ancient or artificially aged materials) for the study of cultural heritage.

Lambda 950 Perkin Elmer

Dual-beam UV-Vis-NIR spectrophotometer for transmission and reflection optical spectroscopy measurements of solid and liquid samples.
Technical specifications:
● spectral range: 200 nm – 3300 nm;
● double monochromator (each equipped with two holographic gratings);
● bandwidth: 0.05 – 5.00nm (UV/Vis), 0.2 – 20 nm (NIR);
● detection system: two detectors (PMT, PbS), with a photometric limit: 8 absorbance.
Available configurations:
● diffuse light intensity measurements in transmission and/or reflection with a 6 cm diameter integrating sphere (spectral range: 250 nm – 2500 nm, photometric limit: 2/3 absorbance) equipped with two detectors (PMT and PbS);
● can be coupled with optical fibres for the study of absorption spectra;
● transmission measurements from ambient T to 15 K with cryostat.
Applications:
● study of glass and ceramic materials for applications in photonics and microelectronics;
● characterisation of liquid suspensions of nanostructured materials;
● study of the colour of stones of gemmological interest.

 

Lambda 900 Perkin Elmer

Dual-beam UV-Vis-NIR spectrophotometer for transmission and reflection optical spectroscopy measurements of solid and liquid samples.
Technical specifications:
●spectral range: 200 nm – 3300 nm;
● double monochromator: each equipped with two holographic gratings;
● bandwidth: 0.05 – 5.00nm (UV/Vis), 0.2 – 20 nm (NIR);
● detection system: two detectors (PMT, PbS), with a photometric limit: 6 absorbance.
Available configurations:
● transmission measurements at normal and oblique incidence (angle of incidence: -75°/+75°);
● absolute specular reflectivity measurements at near- normal incidence (incidence angle ~ 7.5°);
● specular reflectivity measurements relative to oblique incidence (angle of incidence: 15°/60°);
● measurements in linearly polarised light (spectrophotometer equipped with Glan-Taylor depolariser and polarisers);
● transmission (at normal and oblique incidence) and absolute specular reflectivity (at near-normal incidence) measurements at low temperature (down to 7 K);
● transmission and/or reflection scattered light intensity measurements with a 15 cm diameter integrating sphere (spectral range: 250 nm – 2500 nm, photometric limit: 2/3 absorbance) equipped with two detectors (PMT and PbS).
Applications:
● optical characterisation of inorganic powders;
● study of organic and inorganic solids, whether crystalline, amorphous or nanostructured;
● optical characterisation of samples (e.g. pigments, ancient or artificially aged materials) for the study of cultural heritage.

Microvolume spectrophotometer

NanoDrop One C Thermo Fisher Scientific

The NanoDrop OneC spectrophotometer is a compact instrument developed for microvolumetric analysis, equipped with a patented self-correcting optical path system for analysis both concentrated and dilute solutions.
Technical specifications:
● spectral range: 190-850 nm;
● small sample quantities required: 1-2 µL.
Available configurations:
● cuvette holder for analysis of ultra-dilute samples and for kinetic studies with the possibility of heating and/or stirring the samples.
Applications:
● evaluation of peptides, nucleic acids (DNA, RNA), proteins;
● toxicological tests, dyes, gold nanoparticles;
● colorimetric assays to determine protein concentration (BCA, Bradford, modified Lowry, Pierce);
●optical density measurements.

Spectropolarimeter

CD J815 Jasco

Circular dichroism spectropolarimeter for studying the intrinsic or induced chiral properties of biomolecules.
Technical specifications:
● spectral range: 163 – 900 nm;
● sample temperature: controlled from -10°C to +110°C by a Peltier element.
Available configurations:
● accessory for coupled measurements in circular dichroism and fluorescence.
Applications:
● structural properties of biomolecules;
● protein secondary and tertiary structures;
● conformational stability of proteins;
● comparison with RLD (reference listed drug);
● study of protein-ligand, protein-DNA interaction etc…
CD spectroscopy provides complementary information to other analytical techniques. For example, the conformational properties and stability of peptides and proteins can be studied using CD, FTIR spectroscopy, and intrinsic and extrinsic fluorescence. Additionally, for studying biomolecule interactions, CD spectroscopy is complementary to other methods such as surface plasmon resonance (SPR) spectroscopy, fluorescence, and calorimetry (Isothermal Titration Calorimetry).

Contact us

If you believe these techniques could be useful for characterizing, updating, or designing your materials, please feel free to contact us